Biology: Dr. Geusz
Dr. Michael E. Geusz


Dr. Michael E. Geusz

Ph. D., Vanderbilt University
Office:     414 Life Sciences Building
Phone:   1-419-372-2433
Genes controlling circadian rhythms of neurons and cancer cells
Research and Personal Page


Research Interests:

The primary interest of the laboratory is the organization of the circadian timing system of mice as a model for studying how circadian rhythms in behavior and physiology affect cancer and other diseases.The interactions between the circadian clocks of the nervous system that generate near 24-hour rhythms and the tissues of several target organs are being examined by imaging rhythms in gene expression of whole mice and tissue explant cultures. These cycles are recorded using bioluminescent transgenic mice containing the firefly luciferase gene controlled by immediate-early gene promoters of mPer1, c-fos, and the major immediate-early gene of the human cytomegalovirus. Long-term imaging identified coupled circadian rhythms within the pancreas and cornea and others in midbrain nuclei controlling feeding behavior. These gene expression assays in live cells are combined with pharmacology, histology, neural recordings, and behavioral monitoring to identify the signaling mechanisms acting between the circadian clocks of the nervous system and the body.

Selected Publications:

Sharma VP, Anderson NT, Geusz ME. 2013. Circadian properties of cancer stem cells in glioma cell cultures and tumorspheres. Cancer Lett. Dec 11. pii: S0304-3835(13)00817-3. doi: 10.1016/j.canlet.2013.11.009. [Epub ahead of print] PMID: 24333739

Almanaa TN, Geusz ME, Jamasbi RJ. 2013. A new method for identifying stem-like cells in esophageal cancer cell lines. J Cancer. 4:536-48. PMID: 2398381

Almanaa TN, Geusz ME, Jamasbi RJ. 2012. Effects of curcumin on stem-like cells in human esophageal squamous carcinoma cell lines. BMC Complement Altern Med. 12:195.

Yan, D., Geusz, M.E., and R.J. Jamasbi. 2012. Properties of Lewis lung carcinoma cells surviving curcumin toxicity. Journal of Cancer 3:32-41.

Geusz, M.E., K.T. Blakely, D.J. Hiler, and R.J. Jamasbi. 2010. Elevated mPer1 gene expression in tumor stroma imaged through bioluminescence. International Journal of Cancer 126:620-30.

Hiler, D. J., Bhattacherjee, A, Yamazaki, S, Tei, H, and Geusz, M. E. 2008. Circadian mPer1 Gene Expression in Mesencephalic Trigeminal Nucleus Cultures. Brain Research 1214: 84-93.

Hiler, D.J., Greenwald, M.L., and Geusz, M.E. 2006. Imaging gene expression in live transgenic mice following oral luciferin administration. Photochemical and Photobiological Sciences 5: 1082-5.

Collaco A.M., S. Rahman, E.J.Dougherty, B.B. Williams and M.E. Geusz. 2005. Circadian regulation of a viral gene promoter in live transgenic mice expressing firefly luciferase. Molecular Imaging and Biology 7: 342-350.

Collaco, A.M. and M.E. Geusz. 2003. Imaging whole body immediate-early gene expression in haired and hairless transgenic mice. BMC Physiology 3: 8-19.

Sigworth, L.A., L. Liao, T.R. Chandler and M.E. Geusz. 2003. Luciferase expression controlled by a viral promoter in a mammalian circadian pacemaker. NeuroReport 14: 443-447.

Geusz, M.E. 2001. Bioluminescence imaging of gene expression in living cells and tissues. In: Methods in Cellular Imaging. Basics of Fluorescence, Fluorophores, Microscopy and Detectors. A. Periasamy, (ed.) Oxford Univ. Press, New York, pp. 395-408.

Nunemaker, C.S., R.A. DeFazio, M.E. Geusz, E.D. Herzog, G.R. Pitts and S.M. Moenter. 2001. Long-term recordings of networks of immortalized gonadotropin-releasing hormone neurons reveal spontaneous episodic patterns of action potential firing. J Neurophysiol 86: 86-93.

Sigworth, L.A., T.R. Chandler, L. Liao and M.E. Geusz. 2001. Luciferase imaging reveals distinct patterns of gene regulation in live brain slices. In: Bioluminescence and Chemiluminescence 2000. J. F. Case, P.J. Herring, B.H. Robison, SHD Haddock, LJ Kricka, PE Stanley, (eds.) World Scientific, Singapore. pp. 185-188.

Tosini, G. S. Doyle, M.E. Geusz and M. Menaker. 2000. Induction of photosensitivity in neonatal rat pineal gland. Proceedings of the National Academy of Sciences (USA) 97: 11540-11544.